Leica-biosystems PELORIS_PELORIS II Manuel d'utilisateur

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Page 1 - Leica PELORIS II

Living up to LifeLeica PELORIS™ Leica PELORIS II™ Rapid Tissue ProcessorUser Manual

Page 2 - Legal Notices

Safety NoticesLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 10General Warnings and CautionsPersons operating the Peloris

Page 3 - Contacting Leica Microsystems

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 100Additional limitations may apply depe

Page 4 - Software Licence Terms

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1016.1.2 Reagent ManagementUse the Reage

Page 5 - 4 Intellectual property

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 102RetortsThe Retorts section of the Rea

Page 6 - 7 Confidentiality and privacy

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1036.2 Control MenuThe control screens a

Page 7 - 9 Indemnity

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 104 The inspection interval for the ext

Page 8 - 12 General

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 105Language and Unit SettingsYou can vie

Page 9 - Safety Notices

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 106Color SchemesThere are two software c

Page 10 - General Warnings and Cautions

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 107 Toggle the Show by time/Show by fre

Page 11 - Electrical Hazards

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 108When an alarm activates the associate

Page 12 - Reagents

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1096.2.7 File TransferThe file transfer

Page 13 - Regulatory Approvals

Safety NoticesLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 11Instrument SetupElectrical HazardsWARNINGDo not use cleanin

Page 14 - Contents

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 110Attach or Remove a USB KeyUse a USB k

Page 15

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 111Transferring ProtocolsThe protocol tr

Page 16

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 112Transferring Instrument Condition Fil

Page 17 - Introduction

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 113 To transfer event details tap Event

Page 18 - 1.2 Using the Software

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1147Cleaning and MaintenanceFollow the maintenance and cleaning instruction

Page 19 - 1.2.1 Basic Operation

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1157.1 Cleaning and Maintenance ScheduleSee the lis

Page 20 - 1.2.2 Navigation

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 116Leica Peloris™ User Maintenance ChartWeek: Seria

Page 21

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1177.2 Daily TasksClean Lids and SealsUse the plast

Page 22 - 1.3 Help

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 118Clean RetortsClean the retort walls daily with a

Page 23 - Hardware

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 119If the retort has a build up of salt precipitate

Page 24 - Emergency Shutdown

Safety NoticesLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 12ReagentsWARNINGLocate the instrument so that either the mai

Page 25 - 2.2 Retorts

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1207.3 Weekly TasksEmpty Condensate BottleEmpty the

Page 26 - 2.2.2 Fill Levels

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 121Wipe the bottom and sides of the chamber with a

Page 27 - Liquid Level Sensors

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1227.4 60–90 DaysChange Carbon FilterChange the car

Page 28 - 2.2.4 Cassette Baskets

Cleaning and MaintenanceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 123Check Lid SealsInspect the retort and wax bath l

Page 29 - High-capacity Baskets

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1248ReferenceThis chapter has useful reference information to help you set

Page 30

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1258.1 Reagent Threshold GuidelinesThe tables in this section list

Page 31 - Spaced Baskets

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1268.2 ProtocolsThe protocols in this section have been developed

Page 32 - 2.3 Wax Bath

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 127The pre-defined protocols are: Factory 1hr Xylene Standard (se

Page 33 - 2.4 Reagent Cabinet

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 128Xylene 2 Hour Pre-defined protocol “Factory 2hr Xylene Standar

Page 34 - 2.4.3 Carbon Filter

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 129Xylene 6 Hour Not a pre-defined protocolXylene 8 Hour Pre-def

Page 35 - 2.5 Touch-screen

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 13Regulatory ApprovalsFCC ComplianceThis device complies with part 15 of th

Page 36

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 130Xylene 12 Hour Pre-defined protocol “Factory 12hr Xylene Stand

Page 37

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1318.2.4 Xylene-free ProtocolsAll the xylene-free protocols below

Page 38 - 2.7 Alarm Connections

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 132Xylene-free 4 Hour Pre-defined protocol “Factory 4 hr Xylene F

Page 39

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 133Xylene-free 8 Hour Pre-defined protocol “Factory 8 hr Xylene F

Page 40 - Running Protocols

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1348.2.5 Cleaning ProtocolQuick Clean Pre-defined protocol “Quick

Page 41 - Run Protocol

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1358.3 Station ConfigurationsUse the station configurations in thi

Page 42 - Run Cleaning Protocol

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 136Xylene-free ConfigurationThis is the suggested station configur

Page 43 - 3.2 Cleaning Protocols

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1378.4 Protocol Step TemperaturesThe Peloris tissue processor uses

Page 44 - Pre-cleaning Purges

ReferenceLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 138Standard ProtocolsXylene-free ProtocolsCleaning ProtocolsCurren

Page 45 - 3.3 Status Screen

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1399TroubleshootingIf you have processing problems, review this chapter to

Page 46 - 3.3.1 Status Area

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 14ContentsLegal Notices . . . . . . . . . . . . . . . . . . . . . . . . .

Page 47 - Retort Icons

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 140These questions should help you determine what has gone w

Page 48

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1419.2.1 Under-processed Tissue – Instrument SetupUnder-proc

Page 49 - 3.3.2 Protocol Panels

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1429.2.2 Over-processed Tissue – Instrument SetupOver-proces

Page 50 - 3.4 Protocol Run Options

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1439.2.3 Under- or Over-processed Tissue – ReagentsAretherea

Page 51

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1449.2.4 Poor Processing – Incorrect ProtocolPoor processing

Page 52

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1459.2.5 Poor Processing – Correct ProtocolPoor processing –

Page 53

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1469.2.6 Cutting ArtifactIs the tissue hard, brittle or has

Page 54

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1479.2.7 Staining Artifact Is there cellular detail?Under/Ov

Page 55 - Access Retort

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1489.2.8 Block ArtifactSoft tissue Tissue shrinkage Sweating

Page 56 - 3.6 Retort Scheduling

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 1499.3 Reprocessing RecommendationsNot all problem tissue re

Page 57 - 3.6.3 Unavailable Reagents

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 153.5 Pausing and Abandoning Protocols. . . . . . . . . . . . . . . . . . .

Page 58 - Protocol Setup

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 150 20 mL 5% sodium carbonate Ethanol-glycerol 90 mL 60%

Page 59 - 4.1.1 Protocol Types

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 151C. Slow Reverse ProcessProcess using a modified cleaning

Page 60 - Station Selection Rules

TroubleshootingLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 152Rapid Reverse Cleaning ProtocolSlow Reverse Cleaning Prot

Page 61 - 4.1.3 Pre-defined Protocols

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 15310Consumables and AccessoriesThe following consumable items and accessor

Page 62 - 4.1.5 Carryover Setting

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 15411SpecificationsOperatingDimensions (H x W x D): ...

Page 63

SpecificationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 155EnvironmentalMaximum ambient temperature: ...

Page 64 - Other Products

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 156IndexAabandoning protocols 54access level 22setting 108accessories 153a

Page 65 - 4.1.6 Saving Protocol Files

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 157Event log screen 106event severity & color codes 107external vapor f

Page 66 - 4.2.2 Editing Protocols

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 158protocolabandoning 54backup 111cleaning 43, 59conventional 59copying 71d

Page 67

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 159retort 25acid clean 123cleaning 118cleaning the lids 117default scheduli

Page 68 - Selecting Reagents

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 166.2 Control Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Page 69 - Deleting a Protocol

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 160Wwaxcleaning 102heat lines 99melt temperature 102preheat 100standby temp

Page 70 - 4.2.3 Creating New Protocols

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 171IntroductionThe Leica Peloris™ rapid tissue processor is a dual retort t

Page 71 - 4.2.4 Viewing Protocols

IntroductionLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 181.1 Summary of Chapters Chapter 1, Introduction: general int

Page 72 - Reagent Setup

IntroductionLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 191.2.1 Basic OperationButtonsTap buttons to open menus, screen

Page 73

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 2Legal NoticesIntended Use StatementThe Peloris™ dual retort rapid tissue p

Page 74 - Stations

IntroductionLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 20KeypadsKeypads appear as needed to allow you to enter text an

Page 75 - Managing Concentrations

IntroductionLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 21The table below lists all the screens in the Peloris software

Page 76 - 5.1.3 Thresholds

IntroductionLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 221.2.3 Access LevelsPeloris has two user access levels availab

Page 77 - Temperature Thresholds

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 232HardwareThe Peloris™ tissue processor is a free-standing processor with

Page 78 - 5.1.4 Recommended Reagents

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 24This chapter describes the main features of Peloris instrument.Se

Page 79 - 5.1.5 Tissue Marking

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 252.2 RetortsThe Peloris tissue processor has two independent proce

Page 80 - 5.2 Managing Reagent Types

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 26Some instruments have retort latch locks to disallow opening reto

Page 81

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 27well above the markings, but below the maximum (MAX) level. Reage

Page 82

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 282.2.3 Magnetic StirrerEach retort has as magnetic stirrer that st

Page 83

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 29Opening and Closing Cassette BasketsThe lid is held by two catche

Page 84 - 5.3 Managing Reagent Stations

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 3Contacting Leica MicrosystemsFor service or support contact your local rep

Page 85

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 30cassettes can be placed in the end sections of each row and five

Page 86 -  Setting Station States

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 31Spaced BasketsThe spaced baskets include a fixed divider that ens

Page 87 - Setting Station States

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 322.3 Wax BathThe wax bath at the top rear of the instrument has fo

Page 88 - 5.4 Replacing Reagents

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 332.4 Reagent CabinetFigure 17. Reagent cabinetThe reagent cabinet

Page 89

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 342.4.1 Reagent BottlesThe reagent cabinet holds sixteen reagent bo

Page 90

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 352.5 Touch-screenA touch-screen mounted on the right of the Pelori

Page 91

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 36 For instructions on changing to an external vapor system, see C

Page 92 -  The Remote icon

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 37Returning to the Internal Filter SystemUse the following procedur

Page 93

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 382.7 Alarm ConnectionsFigure 21. Remote alarm (1) and local alarm

Page 94

HardwareLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 39Pin Schematic During Normal OperationWhen the instrument is opera

Page 95 - 5.4.5 Replacing Wax

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 4Software Licence Terms1 Defined terms & interpretation1.1 Defined term

Page 96 -  The Wax waste icon

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 403Running ProtocolsThis chapter describes how to run protocols on the Pelo

Page 97

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 412. Check that the instrument is ready:(i) All 16 bottles

Page 98 - 6.1 Reagents Menu

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 4211. View the progress of the protocol in the Status scre

Page 99 - 6.1.1 Manual Operations

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 433.2 Cleaning ProtocolsCleaning protocols clean the retor

Page 100 - Filling and Draining Retorts

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 44Cleaning Laboratory ImplementsYou can clean metal labora

Page 101 - 6.1.2 Reagent Management

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 45In general, avoid this situation by always cleaning the

Page 102 - Global Wax Settings

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 463.3.1 Status AreaThe status area provides a visual guide

Page 103 - 6.2 Control Menu

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 47Wax Chamber IconsFigure 27. Wax chamber icons on the St

Page 104 - Manual Drain Drip Time

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 48There are ten operations reported for the retorts: Read

Page 105 - 6.2.2 Device Settings

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 493.3.2 Protocol PanelsPanels on each side of the Status s

Page 106 - 6.2.4 Event Log

Software Licence TermsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 52 Grant of licence2.1 Licensee gives consentThe Lice

Page 107 - 6.2.5 Alarms

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 503.4 Protocol Run OptionsYou can schedule each protocol r

Page 108 - 6.2.6 Access Level

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 51How To Schedule a Protocol RunWhen the Scheduling dialog

Page 109 - 6.2.7 File Transfer

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 52Entering a New End Time or Default Scheduling SettingCha

Page 110 - Attach or Remove a USB Key

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 533.4.2 Editing the Protocol for a Single RunYou can edit

Page 111 - Transferring Protocols

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 543.5 Pausing and Abandoning ProtocolsTo stop a protocol t

Page 112

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 55Emergency Stop and AbandonAfter you have paused the inst

Page 113 - Archiving Log Files

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 563.6 Retort SchedulingThe Peloris tissue processor allows

Page 114 - Cleaning and Maintenance

Running ProtocolsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 573.6.3 Unavailable ReagentsOnce a protocol starts, group

Page 115 - As Required

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 584Protocol SetupProtocols control all aspects of the tissue processing run

Page 116

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 59Other protocol properties are the reagent selection method

Page 117 - 7.2 Daily Tasks

Software Licence TermsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 6(b) provide promptly, at its cost, all assistance re

Page 118 - Clean Retorts

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 604.1.2 Reagent Selection MethodAll protocols use one of thre

Page 119 - Clean Touch-screen

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 61Reagent Selection Methods ComparedWhen creating protocols d

Page 120 - 7.3 Weekly Tasks

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 62Pre-defined protocols can be copied to appear in the Protoc

Page 121 - Clean Exterior Surfaces

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 63Carryover has three main components: Reagent on retort wal

Page 122 - 7.4 60–90 Days

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 64run uses the respective cassette or pad type.Sample Calcula

Page 123 - 7.5 Retort Acid Clean

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 654.1.6 Saving Protocol FilesProtocols are saved in “cfg” ext

Page 124 - Reference

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 66Protocol IconsOn the Protocol selection screen each protoco

Page 125 - Xylene-free Processing

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 67Figure 39 and Figure 40 show the Edit protocol screen and d

Page 126 - 8.2 Protocols

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 68Selecting ReagentsWhen you select the Reagent cell in the p

Page 127 - 8.2.3 Xylene Protocols

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 69Station Reagent SelectionIf the protocol uses station reage

Page 128 - Xylene 4 Hour

Software Licence TermsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 78 Exclusions and limitations8.1 AcknowledgmentsThe L

Page 129 - Xylene 8 Hour

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 704.2.3 Creating New ProtocolsCreate new protocols from scrat

Page 130 - Xylene 12 Hour

Protocol SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 71Copying ProtocolsYou can copy any protocol displayed on the

Page 131 - 8.2.4 Xylene-free Protocols

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 725Reagent SetupThis chapter gives an overview of Peloris reagent managemen

Page 132 - Xylene-free 6 Hour

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 735.1.1 Reagent Groups, Types and StationsThe Peloris tissue p

Page 133 - Xylene-free 12 Hour

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 74Reagent type names do not affect reagent concentration. For

Page 134 - 8.2.5 Cleaning Protocol

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 75 Absolute ethanol (100% dehydrant) contaminated by carryove

Page 135 - 8.3 Station Configurations

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 765.1.3 ThresholdsEach reagent type has a range of thresholds

Page 136 - Xylene-free Configuration

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 77Stations with reagent that has exceeded its change threshold

Page 137 - Manual Operations

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 785.1.4 Recommended ReagentsWhile each laboratory is responsib

Page 138 - Cleaning Protocols

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 795.1.5 Tissue MarkingSome laboratories have traditionally add

Page 139 - Troubleshooting

Software Licence TermsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 8(e) any failure by the Licensee to comply with labor

Page 140 - 9.2 Flowcharts

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 805.1.6 Reagent CompatibilityAs tissue processing requires the

Page 141

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 815.2.2 Editing Active Reagent TypesUse the Reagent types scre

Page 142

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 82Figure 45. Reagent types screen, temperature thresholds vie

Page 143

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 835.2.3 Managing the Reagent Types ListsApart from editing the

Page 144

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 845.3 Managing Reagent StationsThe Peloris processor has 20 st

Page 145

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 85See Figure 47 and Figure 48 for descriptions of the informat

Page 146 - 9.2.6 Cutting Artifact

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 865.3.2 Setting Reagent Station PropertiesSee sections: Assig

Page 147 - 9.2.7 Staining Artifact

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 87Changing Reagent ConcentrationYou can set a station’s “calcu

Page 148 - 9.2.8 Block Artifact

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 885.4 Replacing ReagentsAlways replace reagents as soon as pos

Page 149

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 89Figure 50 shows the Remote fill/drain screen with the main f

Page 150 - B. Rapid Reverse Process

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 9Safety NoticesThe Peloris™ tissue processor is designed to provide safe, t

Page 151 - D. Direct Reprocessing

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 90To fit the hose, open the upper reagent cabinet door, slide

Page 152

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 915.4.3 Replacing Reagent – Remote Fill and DrainYou can drain

Page 153 - Consumables and Accessories

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 924. When prompted, check that the retort lid is closed and th

Page 154 - Specifications

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 9312. The instrument will now fill the bottle(s) via the selec

Page 155 - Electrical

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 945.4.4 Replacing Reagent – ManualTo replace bottles manually

Page 156

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 95With this option you must select the bottle state. The conce

Page 157

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 96Drain1. Connect the wax waste hose and place the end in a su

Page 158

Reagent SetupLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 97Fill7. Fill the wax chamber with molten or solid wax.8. Open

Page 159

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 986Settings & Ancillary OperationsThis chapter describes basic system c

Page 160

Settings & Ancillary OperationsLeica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 996.1.1 Manual OperationsThe Manual oper

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