Leica-biosystems ASP200 S Manuel d'utilisateur

Instructions for UseLeica ASP200 S -Advanced Smart ProcessorVacuumTissue ProcessorLeica ASP200 S, V 1.4 RevE, English – 03/2013Order No.: 14 0480 8010

10Instructions for Use V 1.4, RevE – 03/20132. SafetyTransport and InstallationWarnings – Markings on the instrument itselfMarkings on the instrument

11Leica ASP200 S2. SafetyWarnings – Operating the instrumentThe instrument may only be operated by trained laboratory personnel. It must only be op

12Instructions for Use V 1.4, RevE – 03/20132. SafetyWarnings – Handling reagentsWarnings – Cleaning and maintenanceTake care when handling solvents!A

13Leica ASP200 S2.3 Safety features on the instrumentThe Leica ASP200 tissue processor is equipped with numerous safety func-tions and with sophistic

14Instructions for Use V 1.4, RevE – 03/20133.1 Overview – instrument components3. Instrument Components and Specifications2345136714151617181920212

15Leica ASP200 SOverview – instrument components1 - Basic instrument – processor module2 - Basic instrument – reagent module3 - Wax bath lids4

16Instructions for Use V 1.4, RevE – 03/20133. Instrument Components and Specifications3.2 Specific instrument options• Optionally usable Reagent M

17Leica ASP200 S3. Instrument Components and Specifications3.3 Standard delivery - packing listTo prevent damage to the instrument or specimens, o

18Instructions for Use V 1.4, RevE – 03/20133. Instrument Components and SpecificationsPlease check all delivered parts against the packing list and

19Leica ASP200 S3. Instrument Components and SpecificationsNominal supply voltages: Two factory-preset voltages (not user-adjustable):100 to 120 V

20Instructions for Use V 1.4, RevE – 03/20133. Instrument Components and SpecificationsRetortCapacity: max. 200 cassettesReagent volume: 3.5 l with v

21Leica ASP200 S3.5 Compatible reagents3. Instrument Components and SpecificationsHardware and Software:• LargecolorLCDtouchscreen.• User-fri

22Instructions for Use V 1.4, RevE – 03/20133. Instrument Components and SpecificationsThe following reagents may be used in the Leica ASP200 S:Fixat

23Leica ASP200 S3.6.1 Cycle for changing reagentsReagent Week 1 Week 2 Week 3 Week 4Formalin At least 3 times a weekAt least 3 times a weekAt least 3

24Instructions for Use V 1.4, RevE – 03/20134. Initial Operation4.1.1 Moving the instrumentAfter unpacking the instrument (see Unpack-ing instruction

25Leica ASP200 S4. Instrument Setup4.2 Electrical connection• Checkthevoltagelabel(Fig. 3) on the rear of the instrument to ensure that the

26Instructions for Use V 1.4, RevE – 03/2013• Connect the ASP to a grounded socket using the power cable (37).• Using the jumper cable (38), connect

27Leica ASP200 S4. Instrument SetupFig. 5• Movetheinstrumenttoitsfinalsetuplocation.Active carbon filter• Unpacktheactivecarbonfilter

28Instructions for Use V 1.4, RevE – 03/2013Fig. 8Inserting the volume replacement body2625272519Important!The instrument must be operated only when t

29Leica ASP200 S4. Instrument SetupFig. 9Printer (optional)• Swingthedooroftheinstrumentconsole(6) upwards.• Connecttheprintertothe

3Leica ASP200 SThe information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge

30Instructions for Use V 1.4, RevE – 03/20134. Initial OperationChanging the clip• Oncetheclipisinstalled,thehandle(4) of the speci-men baske

31Leica ASP200 S4. Instrument SetupRemote alarmThis alarm is also external to the Leica ASP200 S.If installed, it might typically be connected to a

32Instructions for Use V 1.4, RevE – 03/20134.7 Switching the instrument on4. Initial Operation• Afterbeingswitchedon,theinstrumentwilltake

33Leica ASP200 SSYSTEM DIAGNOSTICS menuIf TOUCH HERE ... is touched during initialization (Fig. 14) and the pass-word for supervisor mode is en-tered,

34Instructions for Use V 1.4, RevE – 03/20134.8 Touchscreen functionsThe Leica ASP200 S is programmed and operated via a color LCD touchscreen.The in

35Leica ASP200 S4. Instrument Setup4.9 Checklist for initial operationParameter selectionSet warning values for the age of the carbon filter and t

36Instructions for Use V 1.4, RevE – 03/20134.10 Switching off the instrumentIf the instrument must be completely switched off or disconnected from t

37Leica ASP200 S5. Operation5.1 Setting up the instrument parametersThe SYSTEM SETUP screen is divided up into five selection fields:• Programo

38Instructions for Use V 1.4, RevE – 03/2013PROGRAM OPTIONSSelect the options with which programs are executed here. Press a button to change the asso

39Leica ASP200 SINSTRUMENTPress PARAFFIN BATH SET TEMP. to open the input screen (Fig. 30) for the wax bath temperature.Set the temperature according

4Instructions for Use V 1.4, RevE – 03/2013Contents1. Important Information ...

40Instructions for Use V 1.4, RevE – 03/2013SECURITYAccess rights to the instrument are managed here as user profiles.5. OperationSUPERVISOR MODE: En

41Leica ASP200 S5. OperationIn the Leica ASP200 S, there is a lock mode to protect against operation by unauthorized per-sons.Enabled:The LOCK key

42Instructions for Use V 1.4, RevE – 03/20135.1.3 INSTALLATION menu→→Use this menu to set the name of the instrument, the language of the user interf

43Leica ASP200 SSetting the altitude of the installation location (in meters above sea level)This is important as it affects the actual pressure in th

44Instructions for Use V 1.4, RevE – 03/2013Printing run logsPrinting is possible when the PRINT symbol is displayed.Configuring a printerOn the INSTA

45Leica ASP200 S5.1.4 Editing the reagent listAdding new reagents1. Enter the reagent name and allocate the new reagent to the reagent group it belon

46Instructions for Use V 1.4, RevE – 03/2013Entering / modifying reagent thresholdsIf warning thresholds are required for a certain reagent, enter the

47Leica ASP200 SDeleting reagents• HighlightthereagenttobedeletedintheSET UP REAGENTS AND WARNING THRESHOLDS screen.• PressCLEAR.• Pres

48Instructions for Use V 1.4, RevE – 03/20135.1.5 Viewing the program listYoucancreate:• Upto15infiltrationprograms• 3retortcleaningprogra

49Leica ASP200 S5.1.6 Adding and/or modifying programsCreating a new program• Makesureyouareloggedonatsupervisorlevel.• IntheVIEW/EDIT P

5Leica ASP200 SContents5.1.4 Editing the reagent list ...

50Instructions for Use V 1.4, RevE – 03/20135. OperationEditing program steps• Toeditastep,highlightthecorrespondinglineandpresstherespec

51Leica ASP200 SEditing program steps (continued)Setting a delay stepA delay step is a step that is extended to ensure that a program will finish at a

52Instructions for Use V 1.4, RevE – 03/20135.1.7 FavoritesUp to five favorites can be configured in the ASP200 S. Favorites can be programmed so that

53Leica ASP200 SAssigning a day for the end timeIf an end time has been assigned to a Favorite program, a specific weekday can also be set on which th

54Instructions for Use V 1.4, RevE – 03/20135.1.8 Configuring the stationsAdding/changing a reagentSelect the desired station. Either by pressing the

55Leica ASP200 S5.1.9 Reagent groupsFig. 53Changing the color of a reagent group• Highlightthelinecontainingthereagenttobemodified.• Presst

56Instructions for Use V 1.4, RevE – 03/20135. Operation5.2 Reagent handling5.2.1 Draining/filling reagents (other than paraffin)1. Manually changin

57Leica ASP200 S5. OperationRemote draining• Onthescreen,selectthereagentcontainer(No. 3) and the receptacle (External). Then press STATI

58Instructions for Use V 1.4, RevE – 03/20135. Operation5.2.2 Replacing the paraffinDraining the paraffin• Connect the paraffindrainhose (36) to

59Leica ASP200 SFilling with paraffin pellets5. OperationFig. 60• Filltheparaffinreservoirtothebrim(Fig. 60, 1) with paraffin pellets. This

6Instructions for Use V 1.4, RevE – 03/20131. Important Information1.1 Symbols in the text and their meanings(5)ENTERWarnings appear in a gray box

60Instructions for Use V 1.4, RevE – 03/2013Filling with liquid paraffinSoftware controlIf the instrument has been filled with liquid paraffin, the in

61Leica ASP200 S5.3 Running programs There are two different windows from which a program can be started:In the Start screen, press the ALL

62Instructions for Use V 1.4, RevE – 03/20135.3.1 Editing a program that has been called up. Fig. 65To modify a program before the start, press the ED

63Leica ASP200 SThe RUN OPTIONS function displays the options with which the current program is started.• LEVEL TEST enabled: The lower level sensor

64Instructions for Use V 1.4, RevE – 03/20135.3.2 Starting a programFig. 69↓→→5. OperationFig. 70• PressingSTART starts the program. Confirm the pro

65Leica ASP200 S5. Operation5.3.3 Opening the retort during a program interruptionFig. 72 Fig. 73Fig. 71• After the signal tone stops, turn the le

66Instructions for Use V 1.4, RevE – 03/20135. Operation5.3.4 Retort emergency release (continued)Fig. 74• Turn the retort lock further clockwise us

67Leica ASP200 S5.4 Reagent statusThis window (Fig. 77) is used to display and update the current reagent status of the stations. In addition, reagen

68Instructions for Use V 1.4, RevE – 03/20135. Operation5.5 System monitorThe SYSTEM MONITOR function provides important infor-mation about the statu

69Leica ASP200 S5.6 The online helpThe Leica ASP200 S has an extensive help system that can be started from every main window.To obtain information a

7Leica ASP200 S1.1 Symbols in the text and their meanings (continued)1. Important InformationSNREFSerial numberOrder No.Observe the Instructions f

70Instructions for Use V 1.4, RevE – 03/20136. Troubleshooting6.1 General→For all error states, the instrument takes corrective measures to protect th

71Leica ASP200 S6. Troubleshooting6.3 Troubleshooting→Use the SMART SCREEN functions to individually test operations such as fill, drain, pressuriz

72Instructions for Use V 1.4, RevE – 03/20136. Troubleshooting6.4 Typical fill or drain problemsFill and drain problems may be due to several causes:

73Leica ASP200 S7. Cleaning and Maintenance7.1 Clean programsThe cleaning programs are at the end of the list of all pro-grams (Fig. 45). To displ

74Instructions for Use V 1.4, RevE – 03/20137. Cleaning and MaintenanceEditing steps• To edit a program step, highlight the corresponding line and p

75Leica ASP200 S7.1.2 Paraffin cleaningIt is possible to clean used paraffin of solvent residues (xylene) in the Leica ASP200 S. To do so, select the

76Instructions for Use V 1.4, RevE – 03/20137. Cleaning and maintenance7.1.3 Smart cleanThe smart cleaning prepares the ASP200 S for transport. Durin

77Leica ASP200 S7.2 General cleaning stepsParaffin reservoirs7. Cleaning and MaintenanceRegularly inspect the collecting tray (16) for signs of le

78Instructions for Use V 1.4, RevE – 03/20137. Cleaning and MaintenanceReplacing the active carbon filter• Thelifeoftheactivecarbonfilterwill

79Leica ASP200 SCleaning the retort lid• Removewaxfromtheinsideoftheretortlidwiththeplasticscraperprovided. Thoroughly remove all wax d

8Instructions for Use V 1.4, RevE – 03/2013The instrument has been designed so that it is safe to use by the operator as well as for pro-cessing speci

80Instructions for Use V 1.4, RevE – 03/20137. Cleaning and MaintenanceCleaning the surface of the infiltration moduleImportant in order to ensure th

81Leica ASP200 SCleaning the reagent containers• Emptyandcleanthereagentcontainers. Using a bottle brush and a laboratory detergent in warm wa

82Instructions for Use V 1.4, RevE – 03/20137. Cleaning and MaintenanceFig. 877.2.2 Periodic cleaning and maintenance (continued)44Fig. 88Fig. 89Use

83Leica ASP200 S7. Cleaning and Maintenance7.3 Checklist for preventive maintenanceStep Daily Weekly Monthly every 3 monthsLubricate the O-rings o

84Instructions for Use V 1.4, RevE – 03/20137. Cleaning and MaintenanceStep Daily Weekly Monthly All 3 monthsCheck the filter screens of the paraffin

85Leica ASP200 S8. Warranty and ServiceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected

86Instructions for Use V 1.4, RevE – 03/2013Appendix 1Notes on Specimen Preparation• Cut the tissue samples that are to be embedded to a size that fi

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9Leica ASP200 S2.1 Safety notes2. SafetyThese Instructions for Use includes important information related to the operating safety and maintenance