Leica-biosystems LN22 Manuel d'utilisateur

Leica LN22Liquid nitrogen freezingdeviceInstructions for UseLeica LN22 – Liquid Nitrogen Freezing DeviceV1.2, English – 10/2012Order No.: 14 0373 8010

10Instructions for Use V1.2, RevB – 10/2012• There are clothing items and protective gearwith certain characteristics which you shouldspecifically avo

11• Once every three months check the actual evaporation rate of yourDewar vessels and compare the measuring results to the evaporationrates specified

12Instructions for Use V1.2, RevB – 10/2012• Never leave LN2 in open containers where it can exchange with theroom atmosphere. The boiling point of LN

13Leica LN22Warnings – Installation/Connection to mainsThe room where the instrument is installed must either be well ventilated or be large enough t

14Instructions for Use V1.2, RevB – 10/2012Warnings – working at the instrumentDuring operation, metal surfaces and components inside the cryochamber

153. Instrument components and specifications3.1 Technical dataGeneralApprovals: The device-specific approval symbols are locatedon the underside of t

16Instructions for Use V1.2, RevB – 10/20123.2 Overview and instrument components3. Instrument components and specifications125341 Cryochamber2 Contro

173. Instrument components and specifications3.3 Standard deliveryThe Leica LN22 standard delivery includes:• Basic instrument (cryochamber)• Dewar,

18Instructions for Use V1.2, RevB – 10/20124.1 Installation site requirements- stable, vibration-free laboratory bench with horizontal and even stagep

19Leica LN224.3 Preparing the microtome forattachment of the LN22• Move the microtome specimen cylinder to therear limit stop.• Pivot the magnifier o

20Instructions for Use V1.2, RevB – 10/20124.4 Installing the cryochamber• Place the cryochamber onto the guide rails (1)on the microtome base plate a

214.5 Connecting to mains4.5.1 Checking the voltage selector settingThe voltage selector is preset to 230V in the factory.Before connecting the instru

22Instructions for Use V1.2, RevB – 10/2012Fig. 164.5.2 Changing the voltage selector setting and inserting the fusesThe voltage selector can be set t

23• Place the tip of the screwdriver into the small recess on the left ofcover (3) and push very carefully, using the screwdriver as a lever, toremove

24Instructions for Use V1.2, RevB – 10/20124.5.5 Connecting the mains cableThe instrument is delivered with various country-specific mains ca-bles.The

25Leica LN22‘TEMPERATURE’ control panel• To indicate the selected temperature value,press the + / - button (4) below the tempera-ture indication brie

26Instructions for Use V1.2, RevB – 10/2012The selected temperature parameters are dis-played for about 4 seconds. Then the displayswitches back to ac

27Leica LN224.8 Specimen holdersDuring operation metal surfaces and components inside thecryochamber can reach temperatures from as low as -196°C up

28Instructions for Use V1.2, RevB – 10/2012Fig. 21Fig. 22• Lock the handwheel of the microtome in12-o’clock position.• Place the specimen holder into

29Leica LN224.8.2 Universal specimen holder and flat specimen holder• Place the specimen into the specimen holder(5) and (6) slightly tighten screw (

3Leica LN22The information contained in this documentationrepresents the state of the art in science andtechnology.We are under no obligation to upda

30Instructions for Use V1.2, RevB – 10/20121 Cryo specimen arm2 Cold bridge3 Clamping screw of the cold bridge4 Specimen holder5 Knife holder6 Knife c

31Inserting the knife holderAlways attach the specimen holder before inserting the knifeholder to avoid damaging the knife!To insert the knife holder

32Instructions for Use V1.2, RevB – 10/20124. StartupFig. 26Adjusting the knife holder laterally:• Release the clamping screws (10, Fig. 24) of the kn

334.10 Preparing the pumping systemUse caution when handling liquid nitrogen!Prior to filling the Dewar vessel with LN2, please refer to the safetyins

34Instructions for Use V1.2, RevB – 10/20124.12 Refilling the Dewar vesselUse caution when handling liquid nitrogen!Please refer to the safety instruc

35Leica LN222. Remove the pump from the Dewar, cover thepump immediately with the yellow protectioncap, place it on the corresponding supportingdevic

36Instructions for Use V1.2, RevB – 10/20125.1 CleaningDo not use acetone or xylene to clean the lacquered surfaces of theinstrument.The cryochamber s

37Leica LN22• Vacuum sectioning debris that sticks to cham-ber walls and bottom or remove it with ben-zine.Caution: flammable!• All removable parts c

38Instructions for Use V1.2, RevB – 10/20125. Cleaning and maintenance• Use one of your hands to hold the cable loopon the chamber bottom while insert

39Leica LN225.2 Maintenance5.2.1 General maintenance instructionsOnly authorized Leica service engineers may open the instrumentfor maintenance and r

4Instructions for Use V1.2, RevB – 10/2012Table of contents1. Important information ...

40Instructions for Use V1.2, RevB – 10/20125.2.3 Exchanging the bulbBefore replacing the lamp, switch off the mains switch and pull themains plug!The

41Fig. 422135.2.4 Removal of the pump valvesThe two lower pump valves (1) and (2) can be easily disassembled forthorough drying. Valve (2) is located

42Instructions for Use V1.2, RevB – 10/20126.1 Ordering informationKnife holdersKnife holder for triangle glass knife and diamond knife ...

43Leica LN227. Warranty and serviceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractualproduct delivered has been subjected to a co

44Instructions for Use V1.2, RevB – 10/2012Notes

5Leica LN22Table of contents5. Cleaning and maintenance ...

6Instructions for Use V1.2, RevB – 10/2012ENTER(5)1.1 Symbols in the text and theirmeaningsWarningsappear in a gray box and are marked bya warning tri

7Leica LN22Be sure to comply with the safety instructions and warnings provided in this chapter.Be sure to read these instructions, even if you are a

8Instructions for Use V1.2, RevB – 10/2012Safety measures in cryopreparationProvided that the safety measures outlined in the present chapter areobser

9If an unconscious person remains in such a low-oxygen environment,death may occur.In case of emergency immediately call a physician and an ambu-lance