Leica-biosystems RM2255 Manuel d'utilisateur

Instructions for UseRM2255Rotary MicrotomeInstructions for UseLeica RM2255V 1.8 RevC, English 07/2013Order No. 14 0502 80101 RevCAlways keep this manu

8Instructions for Use, V 1.8 RevC – 07/20132. SafetySafety instructions – working with the instrument• Take care when handling microtome knives and d

9Leica RM22552.3 Integrated safety devices2. Safety31425Handwheel lock (manual operation only)• Tolockthehandwheel,pushthelever(5) outward

10Instructions for Use, V 1.8 RevC – 07/20132. SafetyKnife guard on the knife holderEach knife holder is equipped with a tightly mounted knife guard (

11Leica RM22553.1 Overview – instrument components3. Instrument Components and SpecificationsHandwheel handle with centering functionLeica RM2255K

12Instructions for Use, V 1.8 RevC – 07/20133. Instrument components and specificationsFig. 8Motor protectionfuseHeat sinkVoltage selector switch wit

13Leica RM22553.3 Technical Data3. Instrument Components and SpecificationsGeneralApprovals: The instrument-specific marks are located next to the

14Instructions for Use, V 1.8 RevC – 07/20133. Instrument components and specificationsMicrotomeSectioning thickness setting:Section thickness settin

15Leica RM22554. Instrument Setup4.2 Standard delivery4.1 Installation site requirementsNever operate the instrument in rooms with an explosion h

16Instructions for Use, V 1.8 RevC – 07/20134. Instrument Setup4.3 Unpacking and installationFig. 9Fig. 1012344445• Loosen and unscrew the six uppe

17Leica RM2255654.3 Unpacking and installation (cont.)Fig. 10a7 9• Takeoutthefixingmodule(5). To do so, hold it by the top edge of the module a

18Instructions for Use, V 1.8 RevC – 07/20134. Instrument SetupThe feather key (4) is loosely placed in the hand-wheel shaft (1) and fixed in place w

19Leica RM22554. Instrument Setup222524 2123The voltage selector is located above the main power switch, on the left-rear side of the instru-ment (

20Instructions for Use, V 1.8 RevC – 07/2013Fig. 134. Instrument SetupConnecting the control panelThe connecting cable (15) of the control panel is f

21Leica RM22555. Operation5.1 Operating elements and their functionsThree-digit display for displaying section thickness/trimming sec-tion thickne

22Instructions for Use, V 1.8 RevC – 07/20135. OperationThree-digit display for section thickness/trimming section thicknessGreen LEDLights up when tr

23Leica RM2255Turn the instrument on with the power switch at the rear.This is followed by a beep. The instrument initializes.The display (here only a

24Instructions for Use, V 1.8 RevC – 07/2013Coarse feed functionsThe electric coarse feed at two speeds is used for a rapid movement of the specimen t

25Leica RM2255When continuous feed is selected, the coarse feed buttons have the same functions as in trimming mode. The STEP function is useful for c

26Instructions for Use, V 1.8 RevC – 07/20135. Operation• Tochangethedisplaymode,pushMENU MODE until the LED of the desired mode is illuminated

27Leica RM22555. OperationConfiguring the retraction settings• Tocalluptheretractionsettings,presstheMENU MODE and CLEAR buttons simultane

1Leica RM2255The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge a

28Instructions for Use, V 1.8 RevC – 07/2013Sectioning window settingThe sectioning window function allows to optimally adapt the size of the sectioni

29Leica RM22555. OperationMode selection• Forselectingthemode,presstheCUT MODE button on the control panel until the green LED of the desire

30Instructions for Use, V 1.8 RevC – 07/2013Motorized sectioningIn motorized operation, you can choose between three operating modes:CONT = continuou

31Leica RM22555. OperationIndication of remaining horizontal feedThe visible and audible remaining feed indication feature informs the user during

32Instructions for Use, V 1.8 RevC – 07/2013• UsingtheCUT MODE button, select the desired operating mode, CONT, SINGLE or STEP, on the control pane

33Leica RM2255Directional specimen holder fixtureOrienting the specimenDisplay of the zero positionFor better display of the zero position, the orien-

34Instructions for Use, V 1.8 RevC – 07/20135. OperationFine adjustment of the force balanceIf another accessory of a different weight is mounted on t

35Leica RM2255Setting up the knife holder base• Releasetheclampinglever(50) by rotating it counterclockwise.• Insertthe knife holder base (

36Instructions for Use, V 1.8 RevC – 07/20135. OperationThere are two versions of the specimen holder fixture, one with and one without specimen ori-e

37Leica RM22555. Operation• Theindexmarks(0°,5°and10°)foradjustmentof the clearance angle (59.1) are located on the right side of the kni

2Instructions for Use, V 1.8 RevC – 07/20131. Important Information ...

38Instructions for Use, V 1.8 RevC – 07/20135. Operation5.7 Clamping the specimen• Rotatethehandwheeluntilthespecimenclampisintheuppermost

39Leica RM22555. Operation• Carefullypushinthebladefromaboveorfromthe side. Make sure that the blade is clamped in the center and, most i

40Instructions for Use, V 1.8 RevC – 07/2013• Usingtherotaryknob,settheappropriatesectioningspeed.• UsingtheCUT MODE button, select the CO

41Leica RM22555. Operation5.10 Sectioning• Centerthehandleofthehandwheel.• UsetheTRIM/SECT key to select the sectioning mode. • Adjustth

42Instructions for Use, V 1.8 RevC – 07/20136. Optional Accessories6.1 Assembly for fixture for specimen clamps6.1.1 Rigid fixture for specimen cla

43Leica RM22556.1.3 Fine-directional fixture for specimen clamps• Beforethefine-directionalfixture for speci-men clamps can be mounted, loosen

44Instructions for Use, V 1.8 RevC – 07/20136. Optional Accessories6.1.4 Quick clamping systemIt is used as specimen holder for use with the fine-di

45Leica RM22556.2.1 Standard specimen clampThe standard specimen clamp is available in two sizes: 40 x 40 mm and 50 x 55 mm. They are designed for di

46Instructions for Use, V 1.8 RevC – 07/20136.2.2 Vee insertThe vee insert (70) is mounted in the hole pro-vided in the lower movable jaw of the stan

47Leica RM22556. Optional AccessoriesClamping of foil pieces• Movethemovablejaw(74) to the right as re-quired by turning the setscrew with an

3Leica RM22556.1.2 Directional fixture for specimen clamps ...

48Instructions for Use, V 1.8 RevC – 07/20136560• Pushthelever(60) forwards.• Mountthecassette(65) horizontally or verti-cally as required.•

49Leica RM22556. Optional Accessories• To mount the required insert (89.1-3) turn the clamping ring (90) counterclockwise and remove it.•

50Instructions for Use, V 1.8 RevC – 07/2013Assembly of the super mega-cassette clamp The super mega cassette clamp should preferably be used with

51Leica RM22556. Optional Accessories6.3 Knife holder base and knife holderRepositioning the knife holder baseThe one-piece knife holder base (rig

52Instructions for Use, V 1.8 RevC – 07/2013Inserting the blade, knife holder E and E-TC• Foldknifeguard(9) downward.• Toinserttheblade,flip

53Leica RM22556. Optional AccessoriesLateral displacement (only for knife holder E)The lateral displacement feature of the knife holder base enable

54Instructions for Use, V 1.8 RevC – 07/2013 Knife holder E with a water trough for low-profile bladeFig. 45The knife holder E with a water trough is

55Leica RM22556. Optional Accessories6.3.3 Knife holder N/NZEnlarged detail: Knife inserted and height-adjustedMounting the knife support bar• Pu

56Instructions for Use, V 1.8 RevC – 07/2013Inserting the knife• Rotatetheknurlednuts(48) on the right and left of the knife holder for-ward in o

57Leica RM22556. Optional Accessories6.4 Blades/knives6.4.1 Disposable bladesDisposable blades - low profile (819)80 x 8 x 0.25 mm01 package of 50

4Instructions for Use, V 1.8 RevC – 07/20131. Important Information1.1 Symbols in the text and their meanings(5)RUN/ STOPManufacturerThis product fu

58Instructions for Use, V 1.8 RevC – 07/2013Knife 16 cm, profile d - steelKnife, 16 cm long, profile dNote: Knife case 14 0213 11140 includedOrder No.

59Leica RM22556. Optional Accessories• Removethe twoscrews(1) using a slotted screwdriver and then remove the cover plate (2).• Inserttheba

60Instructions for Use, V 1.8 RevC – 07/2013The freezer pack consists of the freezer plate (98) and the insulation jacket (99). It is for cooling (or

61Leica RM22556. Optional Accessories6.9 Universal microscope carrier 1 - Baseplate with drilling (a)2 - Vertical column with size 8 cheese hea

62Instructions for Use, V 1.8 RevC – 07/20132. Attach vertical column• Insertthecapscrew(b) into the hole of the baseplate from below. Place the

63Leica RM22556. Optional Accessories6.10 Magnifying lens Fig. 64Fig. 66Fig. 65• Openthescrew(3) on the horizontal arm of the microscope carri

64Instructions for Use, V 1.8 RevC – 07/201324566.11 Cold light source 6.12 Fiber-optic light guideFig 70, Leica rotary microtome with installed opt

65Leica RM22556. Optional Accessories6.13 Ordering information Fixture for specimen clamps, rigid, silver ...

66Instructions for Use, V 1.8 RevC – 07/20136. Optional Accessories Special wrench f. EM holder ...

67Leica RM22557.1.1 Error reportsIf the message "E 1" follows the software version indication after switching on the instrument, a loss of

5Leica RM22551.4 Instrument typeAll information provided in these Instructions for Use applies only to the instrument type indicated on the title pag

68Instructions for Use, V 1.8 RevC – 07/201328Problem Possible cause Corrective action• Thecuttingmotorcannotbestarted.1. The EMERGENCY STOP but

69Leica RM22557. Troubleshooting7.2 Possible faultsProblem Possible cause Corrective action1. Thick/thin sections The sections alternate between

70Instructions for Use, V 1.8 RevC – 07/20137. TroubleshootingProblem Possible cause Corrective action• Thebladeisdull.• Thepressureplateisda

71Leica RM22558. Cleaning and Maintenance• Raisethespecimenclamptotheupperendposition and activate the handwheel lock.• Switchtheunito

72Instructions for Use, V 1.8 RevC – 07/2013Take the knife holder apart for cleaning. To do so, proceed as follows:• Foldtheknifeguard(9) and ej

73Leica RM22558. Cleaning and MaintenanceUniversal cassette clamp• Detachcassetteclamp(13) for a thorough cleaning, removing all paraffin resi

74Instructions for Use, V 1.8 RevC – 07/20138.2.2 Maintenance instructionsNote for inspection• Whentheinstrumentreachesapprox.2millionperform

75Leica RM22558. Cleaning and Maintenance97 881110Knife holder E:• Clampinglever(11) for the lateral displace-ment,• T-piece(88) and guide (97

76Instructions for Use, V 1.8 RevC – 07/20139. Warranty and ServiceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual product del

77Leica RM225510. Decontamination CertificateLeica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloch, GermanyPhone: ++49 (0) 6224 143

6Instructions for Use, V 1.8 RevC – 07/20132. Safety2.1 Safety notesThese Instructions for Use include important information related to the operating

78Instructions for Use, V 1.8 RevC – 07/2013Leica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloch, GermanyPhone: ++49 (0) 6224 143 0Fax

www.LeicaBiosystems.comLeica Biosystems Nussloch GmbHHeidelberger Straße 17-19D- 69226 NusslochPhone: +49 - (0) 62 24 - 143 0Fax: +49 - (0) 62 24 - 1

7Leica RM22552. SafetyWarnings – Safety notes on the instrument itselfWarnings – Transport and installationPersonal safety precautionsSafety notes