Leica-biosystems VT1000 P Manuel d'utilisateur

Leica VT1000 PVibratomeOperating ManualLeica VT1000 PV1.0 English - 01/2010Always keep this manual with the instrument.Read carefully before working w

10Operating Manual V 1.0 – 01/20103. Instrument Components and Specifications3.2 Technical dataGeneral Approvals: The instrument-sp

11Leica VT1000 P4. Setup the Instrument4.1 Standard deliveryThe standard equipment of the Leica VT1000 P is available in 3 variants and includes the

12Operating Manual V 1.0 – 01/20104. Setup the Instrument4.2 Installation site requirements• Stable,vibration-freelaboratorybenchwithhorizont

13Leica VT1000 P4. Setup the InstrumentFig. 64.3 Unpacking and setting up the instrument• Firstchecktheshipmentforexternaldamagesuponarriva

14Operating Manual V 1.0 – 01/20104. Setup the Instrument4.4.1 Checking the performance requirementsThe Vibratome VT1000 A is available in different

15Leica VT1000 P4. Setup the InstrumentSevere damage may occur if the in-strument is connected to a power sup-ply voltage other than that to which it

16Operating Manual V 1.0 – 01/2010Fig. 84. Setup the Instrument4.5 Connecting the foot switchThe standard scope of delivery of the "Profes-sion

17Leica VT1000 P4.6 Assembling the "deluxe specimen tray"4. Setup the InstrumentFig. 9The deluxe specimen tray (22, Fig. 11) is assem-bled

18Operating Manual V 1.0 – 01/20104. Setup the Instrument• Insertthedeluxespecimentrayintothein-stalled fixture so that the slotted screw (28

19Leica VT1000 P4. Setup the InstrumentFig. 174.7 Assembling the specimen clamp• Thespecimenclamp(34, Fig. 16) is assem-bled inside the regular

20Operating Manual V 1.0 – 01/20104. Setup the Instrument4.7.1 Inserting the round specimen trayThe round specimen tray (45) can be installed in pla

21Leica VT1000 P4. Setup the Instrument43Fig. 254.7.2 Assembling the directional specimen holder fixtureThe directional specimen holder fixture allo

22Operating Manual V 1.0 – 01/201011Fig. 26Fig. 27134.8 Assembling the magnifier4. Setup the Instrument14• Pullthelampcarrier(10, in Fig. 27) o

23Leica VT1000 P5. Operation5.1 Operating elements and their functions5.1.1 The control panel5.1.2 The operating elementsLight switchDisplayRotary

24Operating Manual V 1.0 – 01/20105. OperationThe operating elements (continued)This switch and its three switch positions (upper, center, lower) con

25Leica VT1000 P5. OperationThe operating elements (continued)Fig. 32AMPLITUDE rotary knobThis knob controls the relative amplitude of the lateral ex

26Operating Manual V 1.0 – 01/20105. Operation5.2.1 Adjusting the blade presentation angle• Theaccessoriesincludeabladeangleindi-cator (46)

27Leica VT1000 P5. OperationFig. 365.2.2 Adjusting the specimen and bladePositioning the specimen in the specimen holderYou can adjust the late

28Operating Manual V 1.0 – 01/20105. OperationIn some cases, e.g. for large specimens, it is necessary to move the blade holder (50) on the mounting

29Leica VT1000 P5.2.3 Adjustable sectioning windowThe VT1000 A features an adjustable sectioning window that you can use you speed up the process of

3Leica VT1000 PNOTEThe information, numerical data, notes and val-ue judgments contained in this manual repre-sent the current state of scientific kno

30Operating Manual V 1.0 – 01/2010Fig. 41Adjustable sectioning window (continued)• First,adjustthefrontlimitswitchthatisonthe front side of

31Leica VT1000 P5. Operation5.2.4 Adjusting the specimen heightThe thickness of sections generated is controlled by the raising of the specimen incr

32Operating Manual V 1.0 – 01/20105. OperationThe Leica VT1000 P offers a wide variety of options for sectioning fixed and unfixed tissue samples of

33Leica VT1000 PWhen the instrument is switched on for the first time, the blade is automatically moved back in operating state "B" and stop

34Operating Manual V 1.0 – 01/2010As a result of the great variety of specimen types, sizes, shapes, states and preparations, etc. that can be encount

35Leica VT1000 P5. OperationDirect mountingSpecimens rigid enough to be held firmly in the specimen holder without damage may be clamped directly. K

36Operating Manual V 1.0 – 01/20105. OperationThe surfaces onto which the specimens are attached must be clean, dry and free of adhesive residue from

37Leica VT1000 P5. OperationIf the specimen requires a larger surface, you can use specimen tray (39053744) or the deluxe specimen bath (39053745).Sp

38Operating Manual V 1.0 – 01/2010Be careful when handling the dispos-able blades or sapphire blade. The cutting edge is extremely sharp and can cause

39Leica VT1000 P5. OperationThe speed and amplitude should be set to "0" initially. Then, advance the blade until it almost touches the sp

4Operating Manual V 1.0 – 01/2010Contents1. Important Notes ...

40Operating Manual V 1.0 – 01/20105.5 Preparing the specimen bathThe purpose of the specimen bath is four-fold: to lubricate the blade dur-ing the se

41Leica VT1000 PAfter the specimen has been trimmed to create a flat specimen surface, the preparations for actual sectioning can be carried out. The

42Operating Manual V 1.0 – 01/2010Sections generated may be retrieved from the specimen bath by various means.For microscopic applications, the sectio

43Operating Manual V 1.0 – 01/20106. Cleaning and Maintenance6.1 Cleaning the instrument• Alwaysremovethebladebeforedetachingthebladeholder

44Operating Manual V 1.0 – 01/2010Specimen bath areaAfter sectioning is completed, the specimen bath, specimen holder and sectioning blade holder shou

45Leica VT1000 P6. Cleaning and MaintenanceWhen used normally, the Vibratome VT1000 P requires only standard maintenance. To ensure trouble-free oper

46Operating Manual V 1.0 – 01/20106.3 Replacing components6.3.1 FuseIf the instrument fails to function completely, first check for power avail-abil

47Leica VT1000 P7. Optional Accessories7.1 Ordering informationSpecimen mounting block, 3 per package ... 390

48Leica VT1000 P8. Warranty and ServiceWarrantyLeica Biosystems St. Louis LLC guarantees that the contractual product delivered has been subjected to

5Leica VT1000 PNotes, i.e. important information for the user, are highlighted in gray and marked by the symbol. Dangers, warnings and cautions

6Operating Manual V 1.0 – 01/2010The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you a

7Leica VT1000 P2. SafetyWarnings – Markings on the instrument itself• Warninglabelsontheinstrumentmarkedwithawarningtriangleindicatethat

8Operating Manual V 1.0 – 01/20102. SafetyWarnings – Operating the instrument• Takecarewhenhandlingdisposablebladesandsapphireblades.Thecu

9Leica VT1000 P3. Instrument Components and Specifications3.1 Overview—instrumentcomponentsFig. 3FusesInstrument feetConnectionPower cableAdjustm