Leica-biosystems SM2010 R Manuel d'utilisateur

Instructions for UseLeica SM2010 RSliding MicrotomeInstructions for UseLeica SM2010 R, V 1.4 RevC – 09/2013, EnglishOrder No.: 14 0508 80101 RevCAlway

8Instructions for Use V 1.4 RevC – 09/20133. Instrument components and specifications3.2 Technical dataGeneral Approvals: The instrument-speci

9Leica SM2010 R3. Instrument Components and Specifications3.3 Instrument specifications• TheLeicaSM2010Risamanuallyoperatedslidingmicrot

10Instructions for Use V 1.4 RevC – 09/20134. Instrument Setup4.2 Site requirements• Stable,vibration-freelaboratorytablewithhorizontal,flat

11Leica SM2010 R4.3 Unpacking• Tolifttheinstrumentfromthebox,holditonthe left and right of the housing (Fig. 5), lift it out of the foam cu

12Instructions for Use V 1.4 RevC – 09/20134. Instrument Setup2526Horizontal alignmentFor safe and accurate work, it is important that all instrument

13Leica SM2010 R4.5 Assembling the knife holder SN• Locktheknifesledge(11) in place using the locking knob (23) so that it cannot be moved.• Th

14Instructions for Use V 1.4 RevC – 09/2013Fastening the knife holder• Presstheclampinglever (66) of the knife holder (67) downwards and screw th

15Leica SM2010 RFig. 13Setting the clearance angle (Fig. 13)• Unscrewtheknurledheadscrew(69).• Settherequiredanglewiththeadjustinglever

16Instructions for Use V 1.4 RevC – 09/20134.6 Assembling the blade holder SE4. Instrument SetupMounting the grooved plate• Locktheknifesledge(

17Leica SM2010 R4. Instrument SetupInserting the blade holder SE• Pushthebladeholderfullytothebacksuchthat the T-piece can be inserted in

18Instructions for Use V 1.4 RevC – 09/20134. Instrument SetupInclination (declination) of the blade holder SE• UnscrewtheAllenscrew(52) at the

19Leica SM2010 RAdjusting the clearance angle and declination• Theindexmarks(0°to10°)foradjustingtheclearance angle (43.1) are on the right

20Instructions for Use V 1.4 RevC – 09/20134. Instrument SetupThe object orientation allows for simple position correction of the specimen surface wh

21Leica SM2010 R5. Operation5.1 Operating elements and their functionsThe section thickness is set by turning the adjust-ing knob (33) on the left

22Instructions for Use V 1.4 RevC – 09/20135. Operation5.1.3 Manual feedThe lever (35) for the manual feed is at the front right of the instrument.•

23Leica SM2010 R5. Operation5.1.5 Directional fixture for specimen clampsOrienting the specimen• Toloosen the clamp, rotatethe eccentricl

24Instructions for Use V 1.4 RevC – 09/20135. Operation5.2 Clamping the specimen in the universal cassette clamp (UCC)23• Movethecassetteclamp(4

25Leica SM2010 R5. Operation5.3 Clamping a disposable bladeInsert the blade (Fig. 28)• Push the knife guard (14) towards the right and push t

26Instructions for Use V 1.4 RevC – 09/20135.4 Replacing the pressure plateThe blade holder SE can be converted from use of narrow blades to use of w

27Leica SM2010 R• Locktheknifesledge(11) in place using the locking knob (23).• Pleasemakesurethattheknifeholderis firmly clamped using t

1Leica SM2010 RLeica Biosystems Nussloch GmbHHeidelberger Str. 17 - 19D –69226 NusslochGermanyPhone: +49 62 24 143–0Fax: +49 6224 143–268 Interne

28Instructions for Use V 1.4 RevC – 09/2013Cutting into the specimen (trimming)For trimming, the specimen feed can be dis-engaged either by turning th

29Leica SM2010 R5.7 Changing the specimen or interrupting sectioning5.8 Finishing daily routine• Raisethespecimentothelowerendpositionbytu

30Instructions for Use V 1.4 RevC – 09/20136.1 Cleaning the instrument6. Cleaning and maintenanceBefore each cleaning carry out the following prepar

31Leica SM2010 RThe instrument is basically maintenance-free. To ensure trouble-free operation of the instrument over a long period of time, the follo

32Instructions for Use V 1.4 RevC – 09/20137.1 Ordering informationSupermega cassette clamp with adapter, silver ...

33Leica SM2010 RSupermega cassette clamp,with adapter, silverfor mounting in the quick change system for specimen imagingMaximum specimen size: 75 x 5

34Instructions for Use V 1.4 RevC – 09/2013Standard specimen clamp,with adapter, silverfor mounting in the quick change system for specimen imagingMax

35Leica SM2010 R7. Optional AccessoriesBlade holder SE, assemblyEasily converted from a low-profile to high-profile blade holder by switching the p

36Instructions for Use V 1.4 RevC – 09/20137. Optional accessoriesKnife holder SN, assemblyfor resharpenable knives or blade rails. Blade holder decl

37Leica SM2010 RPlastic knife guard,for blade railsOrder No. ... 14 0368 337727. Optional AccessoriesFig.

2Instructions for Use V 1.4 RevC – 09/2013Table of contents1. Important Information ...

38Instructions for Use V 1.4 RevC – 09/2013Leica low-profile disposable blades - Type 819Length 80 mm, height 8 mm1 package of 50 piecesOrder No. ...

39Leica SM2010 R8. TroubleshootingIn the following table there is a list of the most common problems which can arise while work-ing with the instru

40Instructions for Use V 1.4 RevC – 09/2013WarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected

41Leica SM2010 R10. Notes

42Instructions for Use V 1.4 RevC – 09/201311. Decontamination Certificate (Master)Leica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloc

43Leica SM2010 RLeica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloch, GermanyPhone: ++49 (0) 6224 143 0Fax: ++49 (0) 6224 143 268www.

www.LeicaBiosystems.comLeica Biosystems Nussloch GmbHHeidelberger Straße 17–19D– 69226 NusslochPhone: +49 - (0) 6224 - 143 0Fax: +49 - (0) 6224 - 143

3Leica SM2010 RSymbols in the text and their meanings1. Important Information(5)Intended use of instrumentThe Leica SM2010 R is a manually operated

4Instructions for Use V 1.4 RevC – 09/20132. Safety2.1 Safety notesThese Instructions for Use include important information related to the operating

5Leica SM2010 R2. SafetyWarnings - Safety instructions / warning labels attached to the instrumentWarnings - Transport and installationWarnings - W

6Instructions for Use V 1.4 RevC – 09/20132. Safety2.3 Integrated safety devicesThe instrument is equipped with the following safety devices:• Knife

7Leica SM2010 R3. Instrument Components and SpecificationsSpecimen cylinderRemovable section waste tray3.1 Overview - instrument componentsKnife